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  • Title: Characterization of antigens from Aspergillus fumigatus. I. Preparation of antigens from organisms grown in completely synthetic medium.
    Author: Kim SJ, Chaparas SD.
    Journal: Am Rev Respir Dis; 1978 Sep; 118(3):547-51. PubMed ID: 101103.
    Abstract:
    Aspergillus fumigatus was found to grow well in completely synthetic asparagine glycerol medium. Four-day-old mycelia were totally disrupted by vigorous mechanical shaking with 0.45-micrometer glass beads. Such mechanically produced extracts contained at least 52 precipitating antigens. The number of protein and carbohydrate components was maximal in 4-day-old organisms and decreased in older cultures. Conversely, the number of components in culture filtrates increased with longer incubation periods, but was only approximately one-half that produced by the 4-day-old mycelial extracts.
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