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  • Title: [Two forms of alkaline phosphatase and 5'-nucleotidase in the serum of persons with jaundice of different origin].
    Author: Radzevich IM.
    Journal: Ukr Biokhim Zh; 1976; 48(6):695-700. PubMed ID: 1014137.
    Abstract:
    Distribution of the alkaline phosphatase and 5'-nucleotidase activity was studied in blood serum by means of gel filtration through Sephadex G-200 with jaundices of different origin. Both enzymes have two forms differing in the molecular weight, 5'-nucleotidase presenting mainly a high-molecular form in contrast to alkaline phosphatase. This form activity for both enzymes is higher with obturative jaundices as compared to liver cirrhosis and virus hepatitis. The results of incubating sera with desoxicholate and the subsequent gel filtration in its presence, as well as polyacrylamide gel electrophoresis of butanol extracts of the fractions containing high-molecular fragments, evidence for the fact that these fragments are lipoproteid complexes.
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