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  • Title: [Detection of ABO genotypes by simultaneous PCR-RFLP method].
    Author: Yang Q, Zhu C.
    Journal: Zhonghua Yi Xue Yi Chuan Xue Za Zhi; 1999 Apr; 16(2):110-2. PubMed ID: 10194259.
    Abstract:
    OBJECTIVE: To study human ABO genotyping by means of multiplex PCR and restriction fragment length polymorphism. METHODS: Two specific fragments of ABO gene were simultaneously amplified in a single tube, and then the double restriction digestion with RE Kpn I and Alu I was performed in the same tube. The amplified products were analyzed by PAGE and silver staining. A total of 125 Han unrelated individuals living in Wuhan were genotyped. RESULTS: Six genotypes of ABO were detected and the distribution was in good agreement with Hardy-Weinberg equilibrium. CONCLUSION: The results demonstrate that PCR-RFLP based approach is convenient, reliable and should be of value in forensic application.
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