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  • Title: Endocrine pancreatic cells from Xenopus laevis: light and electron microscopic studies.
    Author: Lozano MT, Hernández MP, Agulleiro B.
    Journal: Gen Comp Endocrinol; 1999 May; 114(2):191-205. PubMed ID: 10208768.
    Abstract:
    Insulin, glucagon, pancreatic polypeptide (PP), peptide tyrosine tyrosine (PYY), somatostatin (SST)-28 (1-12), salmon (s) SST-25, and SST-14 immunoreactivities were demonstrated in the pancreatic endocrine cells of Xenopus laevis using light and electron microscopic immunocytochemistry. Insulin-, SST-28 (1-12)/SST-14-, and PYY-immunoreactive (ir) cells were found throughout the pancreas either isolated in small clusters of a single cell type or, except in the case of PYY-ir cells, forming islets consisting of various cell types. Anti-sSST-25 serum detected the invariant SST-14 form. Cells that were only immunoreactive to glucagon were isolated or clustered in the duodenal lobe, while in the splenic lobe cells immunoreactive to both glucagon and PP were observed in isolation, clustered, or in the periphery of the islets. There were no cells that were immunoreactive only to PP or to NPY. Ultrastructurally, the endocrine cells were characterized by their secretory granules, which were immunogold labeled with the corresponding antisera. Insulin cells had large round secretory granules with a round, irregular, or crystalline-like dense core. Glucagon-ir cells had round secretory granules with a dense core and a clear halo. Glucagon/PP- and PYY-ir cells showed round, ovoid, or pear-shaped secretory granules, which were larger and less electron dense in the latter cell type. The secretory granules of SST-ir cells were ovoid or bacillary with a medium electron-dense content. A sixth cell type with very small secretory granules could only be characterized by conventional electron microscopy, since it did not immunoreact with any of the antisera applied in this study.
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