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  • Title: Amplification of Proteus mirabilis chromosomal DNA using the polymerase chain reaction.
    Author: Mansy MS, Fadl AA, Ashour MS, Khan MI.
    Journal: Mol Cell Probes; 1999 Apr; 13(2):133-40. PubMed ID: 10208804.
    Abstract:
    A Proteus mirabilis-specific polymerase chain reaction (PCR) was developed and standardized. The origin of the primers was a recombinant clone that contained P. mirabilis-specific Hind III fragment DNA of 3.5-kilobase pairs. Based on the sequence data of P. mirabilis recombinant clone, two primers designated MMKAP 1 and MMKAP 2 were synthesized for use in the PCR. A P. mirabilis-specific 3.5-kb pair DNA product was amplified by the primers from 18 strains of P. mirabilis, but not from other Protease species and bacteria. The minimum amount of target DNA detected by P. mirabilis PCR was 10 fg using ethidium bromide/ultraviolet exposure of gels or Southern blot hybridization with a P. mirabilis recombinant DNA probe.
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