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  • Title: Detection of avian adenovirus by polymerase chain reaction.
    Author: Xie Z, Fadl AA, Girshick T, Khan MI.
    Journal: Avian Dis; 1999; 43(1):98-105. PubMed ID: 10216765.
    Abstract:
    An avian adenovirus-specific polymerase chain reaction was developed. The origin of primers was from the DNA sequence data of the chicken embryo lethal orphan avian adenovirus virus genome. An avian adenovirus-specific 421-bp DNA product was amplified by these primers from group I of adenovirus containing 12 serotypes and serotypes of adenovirus from group II and group III. The adenovirus-specific DNA product was also amplified from the 19 field isolates of avian adenoviruses but not from the mammalian adenovirus and other avian pathogenic viruses and bacteria. As little as 1 fg of avian adenovirus DNA was detected by gel electrophoresis and Southern blot analysis.
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