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  • Title: An assay for mandelate racemase using high-performance liquid chromatography.
    Author: Bearne SL, St Maurice M, Vaughan MD.
    Journal: Anal Biochem; 1999 May 01; 269(2):332-6. PubMed ID: 10222006.
    Abstract:
    Mandelate racemase (EC 5.1.2.2) catalyzes the interconversion of the two stereoisomers of mandelic acid. A fixed-time assay for the quantification of mandelate racemase activity has been developed. The assay involves enzymatic conversion of R-mandelate to S-mandelate (or the reverse reaction) followed by separation and detection of the substrate and product using isocratic reversed-phase high-performance liquid chromatography on a Sumichiral OA-6100 column and absorbance detection. This method offers an economical and efficient alternative to the existing circular dichroism-based and coupled assays.
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