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  • Title: X-ray diffraction from a single layer of purple membrane at the air/water interface.
    Author: Verclas SA, Howes PB, Kjaer K, Wurlitzer A, Weygand M, Büldt G, Dencher NA, Lösche M.
    Journal: J Mol Biol; 1999 Apr 16; 287(5):837-43. PubMed ID: 10222193.
    Abstract:
    X-ray diffraction patterns have been recorded from a single layer of purple membrane ( approximately 50 A thickness) at the air/water interface in a Langmuir trough. Grazing-incidence X-ray diffraction is demonstrated to be a promising method for obtaining structural information on membrane proteins under physiological conditions. The method is so sensitive that diffraction can be measured from samples with only 10(13) protein molecules in the beam. Diffraction from hexagonal crystals of purple membrane with a lattice constant of 61. 3 A was observed up to the order {h,k}={4,3}, corresponding to a resolution of approximately 9 A. The work reported here is a first step towards a new way of protein crystallography using grazing-incidence X-ray diffraction at the air/water interface.
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