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  • Title: The pssB gene product of Rhizobium leguminosarum bv. trifolii is homologous to a family of inositol monophosphatases.
    Author: Janczarek M, Król J, Skorupska A.
    Journal: FEMS Microbiol Lett; 1999 Apr 15; 173(2):319-25. PubMed ID: 10227162.
    Abstract:
    The Rhizobium leguminosarum bv. trifolii region encoding pssA and pssB genes was cloned. The pssB gene located upstream of the pssA encoded a 28.36-kDa protein which displayed 97.5% identity with the PssB of R. leguminosarum bv. viciae. Inactivation of the pssB gene by insertion of the lacZ-Gmr cassette resulted in the significant increased production of exopolysaccharide in comparison to the wild-type level. A mutant strain was also defective in nitrogen fixation suggesting a regulatory role of pssB in symbiosis with clover.
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