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  • Title: [Method of gradient chromatography for the isolation of purified lipid fractions from nerve tissue in studies using radioactive carbon, C14].
    Author: Taranova NP.
    Journal: Vopr Med Khim; 1976; 22(4):567-9. PubMed ID: 1027245.
    Abstract:
    A method is developed for separation of lipids from nervous tissue by thin-layer chromatography in silica gel. Cholesterol and "minor" lipids were removed from the start by the first chromatography in the system ether-benzol-ethanol-acetic acid. Phospholipids and galactolipids, which remained at the start, were separated by the repeated chromatography in the system chloroform-methanol-NH4OH. The purified fractions of cholesterol, cerebrosides, sulfatides and the total fraction of phospholipids were obtained from 100-150 mg of nervous tissue in amounts, sufficient for estimation of their specific radioactivity in experiments with 14C.
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