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  • Title: Faster isolation of recombinant factor VIII SQ, with a superporous agarose matrix.
    Author: Pålsson E, Smeds AL, Petersson A, Larsson PO.
    Journal: J Chromatogr A; 1999 Apr 23; 840(1):39-50. PubMed ID: 10335610.
    Abstract:
    A superporous agarose matrix was compared with a corresponding homogenous matrix in the isolation of recombinant factor VIII SQ (r-VIII SQ) by immunoaffinity chromatography. As a reference, the commercially available Sepharose FastFlow, used for a similar purification in the industry, was also evaluated. Breakthrough curves are described for flows between 50 and 400 cm/h with pre-purified r-VIII SQ and with cell culture broth. The superporous gel gave the best performance and a 1000-fold purification was obtained in a one-step procedure. The superporous matrix made it possible to increase the throughput about four-fold, presumably due to its better mass transfer properties. The importance of the ligand distribution profile is discussed based upon immunofluorescence microscopy data.
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