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  • Title: Identification of bacteria using two degenerate 16S rDNA sequencing primers.
    Author: Boye K, Høgdall E, Borre M.
    Journal: Microbiol Res; 1999 May; 154(1):23-6. PubMed ID: 10356793.
    Abstract:
    Two degenerate 16S rDNA primers have been designed for broad-range identification of eubacteria by PCR and automated sequencing. Using a simple method, the primers have proven useful in identification of proteobacteria (Campylobacter, Enterobacter, Escherichia, Helicobacter, Klebsiella), gram-positive bacteria (Mycobacterium, Staphylococcus, Streptococcus) and spirochetes (Borrelia) derived from clinical samples. In several cases, the samples could be identified at the species level.
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