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  • Title: Isolation and characterization of biologically active lectin from Korean mistletoe, Viscum album var. Coloratum.
    Author: Lee HS, Kim YS, Kim SB, Choi BE, Woo BH, Lee KC.
    Journal: Cell Mol Life Sci; 1999 Apr; 55(4):679-82. PubMed ID: 10357236.
    Abstract:
    A mistletoe lectin was isolated from water extracts of Korean mistletoe, a subspecies of Viscum album, grown on Quercus mongolica using CM-Sepharose chromatography followed by an affinity chromatography on a concanavalin A-Sepharose column. The compound proved to be a mistletoe lectin II with D-galactose and N-acetyl-D-galactosamine specificity. Matrix-assisted laser desorption time-of-flight mass spectroscopy showed it to have an average molecular mass of 62.7 kDa and to consist of two subunits of 30.6 kDa and 32.5 kDa. It was a basic protein with isoelectric points of 9.4 and 9.6 by capillary isoelectric focusing and was cytotoxic to Molt4 cell.
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