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  • Title: [Characteristics of RNA isolated from the nuclear RNA-synthesizing complex of influenza virus].
    Author: Asadullaev TA, Bukrinskaia AG.
    Journal: Vopr Virusol; 1976; (6):656-9. PubMed ID: 1035995.
    Abstract:
    When Ehrlich ascitic carcinoma cells infected with classical fowl plague virus and treated with actinomycin D were pulse labeled for 10 min with 3H-uridine, it was mainly incorporated into nucleoplasm structures sedimenting in sucrose gradients at 120S. At 2-hr exposure of the infected cells to 3H-uridine radioactivity was found in nucleoplasm in the area of 65S and in the cytoplasm in 30-40S zone. The analysis of RNA isolated from these structures gave the following results. The RNA isolated from 120S structures sedimented in two zones of sucrose gradient: 11S and 16-23S. The 11S RNA was resistant to RNA-ase, while 16-23S RNA was sensitive to RNA-ase. A similar (16-23S) RNA was isolated from virus-specific structures 65S and 30-40S.
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