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  • Title: Optimized analysis of intracellular adenosine and guanosine phosphates in Escherichia coli.
    Author: Meyer S, Noisommit-Rizzi N, Reuss M, Neubauer P.
    Journal: Anal Biochem; 1999 Jun 15; 271(1):43-52. PubMed ID: 10361003.
    Abstract:
    To investigate the intracellular concentrations of adenosine phosphates in Escherichia coli, especially during bioreactor cultivations, a method that enables reproducible determination of adenosine phosphates in culture solutions containing at least 0.25 g dry cell weight/L has been developed. The detection limits of AMP, ADP, and ATP were found to be as low as 1 pmol. The method involves fast sampling, instantaneous inactivation of cell metabolism, extraction of nucleotides, and quantitative analysis by ion-pair reversed-phase HPLC.
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