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  • Title: Effects of oxamate, an inhibitor of lactate dahydrogenase, upon the spontaneous or oxytocin-induced motility and over pyruvate levels of uterine horns isolated from ovariectomized or estrus rats.
    Author: Gimeno AL, Goldraij A, Gimeno MF, Santillan de Torres R.
    Journal: Reproduccion; 1976; 3(1-2):5-14. PubMed ID: 1036172.
    Abstract:
    The stability with time of the spontaneous as well as the oxytocin-triggered functional activity of uterine borns isolated from induced estrus rats and immersed in a medium with lactate as the substrate was not affected by the presence of oxomate (an inhibitor of lactate dehydrogenase) at 10 or 20mM. On the contrary, the oxytocin-driven and the spontaneous motility of preparations obtained from 15 days castrated rats diminished significantly following in addition of the enzyme inhibitor. Furthermore, uterine borns from ovariectomized animals injected with 17-beta estradiol, regain the lack of functional sensivity towards oxamate. In addition, determinations of pyruvate levels in homogenates of uterine tissue, previously suspended in lactate medium (as in the functional experiments), demonstrate to be significantly reduced, following oxamate. Several possible reasons, attempting to explain these findings, are discussed. The pharmacological effects of oxamate on the spontaneous or the oxytocin-induced motility of isolated rat uterus in a solution with lactate as the sole external substrate were investigated during estrus, following ovariectomy, and after castration accompanied with estradiol replacement therapy. Uterine preparations were tested for mechanical activity (isometric-developed tension and the frequency of contractions). Initial oxytocin-induced activity of the uterus of rats in induced estrus was significantly greater than spontaneous activity (p.01). Oxamate added to the suspension was ineffective in modifying this activity. In castrate rats, however, the addition of oxamate to the medium significantly reduced oxytocin-driven and spontaneous motility. Those ovariectomized rats receiving replacement therapy were also resistant to oxamate effects. The effects of oxamate upon pyruvate levels of oxytocin-exposed uterine tissue were also determined. In the presence of oxamate, levels of uterine pyruvate in all the hormonal conditions were significantly lower than in controls (p.001).
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