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  • Title: Cloning and expression of Der f 6, a serine protease allergen from the house dust mite, Dermatophagoides farinae.
    Author: Kawamoto S, Mizuguchi Y, Morimoto K, Aki T, Shigeta S, Yasueda H, Wada T, Suzuki O, Jyo T, Ono K.
    Journal: Biochim Biophys Acta; 1999 Jul 07; 1454(2):201-7. PubMed ID: 10381565.
    Abstract:
    House dust mite allergen is thought to be a major cause of asthma. Characterization of these allergen molecules is therefore an important step for the development of effective diagnostic and therapeutic agents against mite-associated allergic disorders. Here we report molecular cloning and expression of the group 6 (chymotrypsin-like) allergen from the house dust mite, Dermatophagoides farinae. Sequencing analysis indicates that cloned cDNA, designated Der f 6, encodes a 279 amino acid polypeptide which conserves a primary structure characteristic for chymotrypsin-like serine proteases found in mammals. Recombinant Der f 6 expressed in Escherichia coli bound IgE in a pool made of 20 sera, and induced histamine release from patients' peripheral blood cells.
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