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  • Title: A stable triple Wolbachia infection in Drosophila with nearly additive incompatibility effects.
    Author: Rousset F, Braig HR, O'Neill SL.
    Journal: Heredity (Edinb); 1999 Jun; 82 ( Pt 6)():620-7. PubMed ID: 10383683.
    Abstract:
    Drosophila simulans strains infected with three different Wolbachia strains were generated by experimental injection of a third symbiont into a naturally double-infected strain. This transfer led to a substantial increase in total Wolbachia density in the host strain. Each of the three symbionts was stably transmitted in the presence of the other two. Triple-infected males were incompatible with double-infected females. No evidence was obtained for interference between modification effects of the different Wolbachia strains in males. Some incompatibility was observed between triple-infected males and females. However, this incompatibility reaction is not a specific property of triple-infected flies, because it was also observed in double-infected strains.
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