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  • Title: A set of plasmids constitutively producing different RNA levels in Escherichia coli.
    Author: Gabriel K, McClain WH.
    Journal: J Mol Biol; 1999 Jul 09; 290(2):385-9. PubMed ID: 10390339.
    Abstract:
    New plasmids were developed for the in vivo expression of RNA in Escherichia coli. These plasmids combine constitutive promoters of different strengths with different origins of replication to provide a 75-fold range of expression of amber suppressor tRNA. The plasmids are either pMB1, p15A or temperature-sensitive SC101 replicons, and can be used in two plasmid systems for studying RNA-protein interactions. The temperature-sensitive SC101 plasmids may be useful as gene replacement vectors. Another vector that is suitable for generating lethal mutations was constructed in a plasmid containing a regulatable phage T7 promoter.
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