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  • Title: Are cumulus cells necessary for the spontaneous maturation of germinal vesicle-stage oocytes to metaphase II.
    Author: Häberle M, Scheurer P, Lauerer K, Fischer M, Hohl MK.
    Journal: J Assist Reprod Genet; 1999 Jul; 16(6):329-31. PubMed ID: 10394530.
    Abstract:
    PURPOSE: In this study we investigated the need of the support from cumulus cells for germinal-vesicle (GV) oocytes collected from stimulated ovaries to complete their maturation to metaphase II (MII). METHODS: We compared the maturation rate of GV oocytes after coculture with cumulus cells (study group) with their spontaneous maturation in culture medium alone (control group). RESULTS: Sixty-four and nine-tenths percent of the GV oocytes matured to metaphase II in the coculture group, and of these, 43.5% gave normal 2pn zygotes following intracytoplasmic sperm injection (ICSI), while 73.8% of the GV oocytes spontaneously matured to the MII stage and 30% of these reached the zygote stage after ICSI. CONCLUSIONS: It is probable that a follicular factor is responsible for this arrested maturation in the human and that maturation occurs spontaneously when the oocytes are separated from their follicular fluid environment after collection.
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