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  • Title: The use of chicken IgY in a double antibody sandwich ELISA for detecting African horsesickness virus.
    Author: Du Plessis DH, Van Wyngaardt W, Romito M, Du Plessis M, Maree S.
    Journal: Onderstepoort J Vet Res; 1999 Mar; 66(1):25-8. PubMed ID: 10396758.
    Abstract:
    An indirect sandwich ELISA that can detect as little as 8 ng of African horsesickness virus (AHSV) was developed. Viral antigen was captured from suspension using an immobilized monoclonal antibody specific for an epitope on VP7, a protein that is a major constituent of the virus core. Egg-yolk derived chicken IgY directed against AHSV (serotype 3) was used as the secondary antibody. Since IgY and mouse IgG do not cross-react serologically, the secondary antibody was not labelled, but was instead detected with enzyme-coupled sheep antibodies directed against avian immunoglobulins. The assay recognized all nine AHSV serotypes, but not the Cascara isolate of equine encephalosis virus, a related orbivirus that also infects horses. In addition to being able to detect and quantify whole AHSV, the ELISA could show the presence of VP7 produced by recombinant baculoviruses.
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