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  • Title: Simultaneous detection of X- and Y-bearing bull spermatozoa by double colour fluorescence in situ hybridization.
    Author: Hassanane M, Kovacs A, Laurent P, Lindblad K, Gustavsson I.
    Journal: Mol Reprod Dev; 1999 Aug; 53(4):407-12. PubMed ID: 10398416.
    Abstract:
    Double colour fluorescence in situ hybridization with sex chromosome probes was applied on sperm cells of five Swedish Holstein-Friesian bulls. It was demonstrated that cosmids with strong fluorescence signals and scraped chromosomes can successfully be used as markers in this type of study. X and Y segregated as expected according to a 1:1 ratio, and there were no interindividual variations. There was a tendency for there to be more Y- than X-bearing spermatozoa, but this bias was assumed to be due to the markers used. Disomic spermatozoa occurred with a frequency of more than 0.1 % (0.067% XX, 0.029% YY, and 0.029% XY), which is considerably lower than the frequency in humans. Diploid sperm cells occurred with a frequency of 0.05 %.
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