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  • Title: Construction of a recombinant herpesvirus expressing the jellyfish green fluorescent protein.
    Author: Boldogköi Z, Erdélyi F, Sik A, Freund TF, Fodor I.
    Journal: Luminescence; 1999; 14(2):69-74. PubMed ID: 10398563.
    Abstract:
    Here we report the insertion of a synthetic version of the cDNA encoding the jellyfish (Aequorea victoria) green fluorescent protein (gfph ) into the genome of pseudorabies (Aujeszky's disease) virus (PrV). A putative latency promoter (PLAT) located at the inverted repeat region of the PrV genome was chosen as the target site for the insertion. Recombinant viral DNA designated as vLAT-gfp was generated as a result of homologous recombination between the transfected viral DNA and a plasmid containing the GFP-expression cassette flanked by viral sequences homologous to the target region. Plaques containing recombinant virus were selected visually using a fluorescent microscope. We demonstrated a GFP-expression in infected neurons of rat brain which showed normal morphology at early stage of viral infection by monitoring fluorescent light emission.
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