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Title: Characterization of Methanosarcina mazei N2M9705 isolated from an aquaculture fishpond. Author: Lai MC, Shu CM, Chiou MS, Hong TY, Chuang MJ, Hua JJ. Journal: Curr Microbiol; 1999 Aug; 39(2):79-84. PubMed ID: 10398831. Abstract: A new methanogenic isolate, designated as strain N2M9705 (=OCM 668), was isolated from an aquaculture fishpond near Wang-gong, Taiwan. This strain grew on trimethylamine and methanol, but it did not catabolize H2-CO2, acetate, or formate. The cells were stained Gram-negative, nonmotile, irregular coccus 0.6-0.8 micrometer in diameter. Gas vacuoles were observed and cell aggregated to form various sizes of granules. Cells grew optimally at 32 degrees -37 degrees C with 1% NaCl. The pH range of growth was 6.2-7.4, and higher pH inhibited the cell growth. The cells grew well in minimal medium, but growth was greatly stimulated by yeast extract and peptone. A comparison of 16S rDNA sequences of this organism phylogenetically related to Methanosarcina mazei. This is the first report of methyltrophic methanogenic isolated from an aquaculture fishpond.[Abstract] [Full Text] [Related] [New Search]