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  • Title: Lim1 related homeobox gene (HpLim1) expressed in sea urchin embryos.
    Author: Kawasaki T, Mitsunaga-Nakatsubo K, Takeda K, Akasaka K, Shimada H.
    Journal: Dev Growth Differ; 1999 Jun; 41(3):273-82. PubMed ID: 10400389.
    Abstract:
    A cDNA clone for the LIM class homeobox gene (HpLim1) of the sea urchin, Hemicentrotus pulcherrimus, was isolated. HpLim1 contains two LIM domains and a LIM-class homeodomain, and amino acid sequences of these three domains are highly homologous to corresponding domains of Lim1 of other animals. Accumulation of HpLim1 transcripts begins at hatching, and declines after the mesenchyme blastula stage. HpLim1 mRNA was localized in the vegetal plates of hatched blastulae, but it was not detectable in primary mesenchyme cells (PMC) ingressed into the blastocele. HpLim1 mRNA-injected embryos became spherical with markedly reduced gut formation, failed to express marker proteins for aboral ectoderm and mesoderm, and mainly expressed an oral ectoderm marker. These results imply that while short-term expression of HpLim1 in the vegetal plate is needed for differentiation of aboral ectoderm, endoderm and PMC, ectopic expression of HpLim1 suppresses normal differentiation directing all embryonic cells to differentiate into oral ectoderm.
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