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  • Title: Glutamate transporter EAAC1 is expressed in neurons and glial cells in the rat nervous system.
    Author: Kugler P, Schmitt A.
    Journal: Glia; 1999 Aug; 27(2):129-42. PubMed ID: 10417812.
    Abstract:
    Oligonucleotide and cRNA probes were used for non-radioactive in situ hybridization, carried out to identify the cell types in the nervous system of rat expressing the glutamate transporter EAAC1 mRNA. The results were compared with immunocytochemical data obtained using an antibody against a synthetic EAAC1 peptide. The present data confirm that EAAC1 is expressed in neurons of the CNS. Additionally, our findings indicate the localization of EAAC1 mRNA and protein in peripheral neurons (spinal ganglia) and in glial cells, i.e., oligodendrocytes in various white matter regions of the CNS, ependymal cells, and epithelial cells of the plexus choroideus of the four ventricles, as well as in satellite cells of spinal ganglia. Immunolabeling revealed a preferentially cytoplasmic staining of neurons and glial cells. The cytoplasmic staining was frequently granular, suggesting a localization of EAAC1 protein in vesicle membranes. A membrane localization of EAAC1 was also indicated by Western blotting, which showed immunoreactivity only in the 100,000 x g pellet of brain homogenate. We conclude that the glutamate transporter EAAC1 is not restricted to neurons but may also play an important role in glial cells, particularly in oligodendrocytes.
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