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  • Title: Using the beta/alpha peak-height ratio of ATP in 31P NMR spectra to measure free [Mg2+]: theoretical and practical problems.
    Author: Mulquiney PJ, Kuchel PW.
    Journal: NMR Biomed; 1999 Jun; 12(4):217-20. PubMed ID: 10421913.
    Abstract:
    A recently described alternative method for determining free [Mg2+] in cells and tissue with 31P NMR spectroscopy, which is based on the peak-height ratio of the beta and alpha resonances of ATP (h(beta/alpha)) [J. Biol. Chem. 271, 21141-21150 (1996)], was found to have numerous problems. These problems include a flaw in the basic multiple equilibrium analysis, the inapplicability of the calibration curve to in vivo studies, and a lack of sensitivity of the measured ratio to changes in free [Mg2+] at 'clinical' field strengths. This suggests that the use of this procedure will be very limited.
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