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  • Title: Selective release, removal, and reconstitution of bacteriochlorophyll a molecules into the B800 sites of LH2 complexes from Rhodopseudomonas acidophila 10050.
    Author: Fraser NJ, Dominy PJ, Ucker B, Simonin I, Scheer H, Cogdell RJ.
    Journal: Biochemistry; 1999 Jul 27; 38(30):9684-92. PubMed ID: 10423247.
    Abstract:
    A method is described which allows the selective release and removal of the Bchla-B800 molecules from the LH2 complex of Rhodopseudomonas acidophila 10050. This procedure also allows reconstitution of approximately 80% of the empty binding sites with native Bchla. As shown by circular dichroism spectroscopy, the overall structures of the B850-only and reconstituted complexes are not affected by the pigment-exchange procedure. The pigments reconstituted into the B800 sites can also efficiently transfer excitation energy to the Bchla-B850 molecules.
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