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  • Title: Serodiagnosis of goat warble fly infestation by Przhevalskiana silenus with a commercial ELISA kit.
    Author: Otranto D, Boulard C, Giangaspero A, Caringella MP, Rimmele D, Puccini V.
    Journal: Vet Rec; 1999 Jun 26; 144(26):726-9. PubMed ID: 10423816.
    Abstract:
    Cross-reactivity between Hypoderma lineatum antigen and anti-Przhevalskiana silenus antibodies has been demonstrated by an ELISA technique. To evaluate the applicability of a commercial ELISA kit for the immunodiagnosis of goat warble fly infestation, different dilutions of serum and conjugate were tested, the development of antibody to P silenus in naturally infested goats was studied, and the results were compared with an ELISA technique using an antigen extracted from the first instar larvae of H lineatum. The best results were obtained with a serum dilution of 1:50; with both techniques the highest antibody concentration were recorded in October, November and December. In view of the confirmed cross-reactivity between H lineatum antigen and anti-P silenus antibodies, and the simplicity and rapidity of the assay, the commercial ELISA kit can be considered as a useful tool for the diagnosis of goat warble fly infestation.
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