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  • Title: Competitive polymerase chain reaction for the determination of N-myc amplification in neuroblastoma: report of clinical cases.
    Author: Hasan Z, Inoue A, Ikeda H, Kamii Y, Obana K, Yokomori K, Tsuchida Y, Hemmi H, Shimatake H.
    Journal: Eur J Pediatr Surg; 1999 Jun; 9(3):138-41. PubMed ID: 10427487.
    Abstract:
    If an unfavorable prognosis is suspected in neuroblastoma, decision on a treatment protocol should be based on the N-myc copy number (12). We already demonstrated that the newly developed competitive polymerase chain reaction (competitive PCR) is a promising method for the determination of the N-myc copy number (6), and have started to use this competitive PCR procedure in neuroblastoma patients, together with fine-needle biopsy in selected cases. Seven children were studied. In one infant of 5 months of age whose tumor was diagnosed before undergoing mass screening for neuroblastoma, the competitive PCR procedure was performed with a fine-needle biopsy, and after obtaining a negative report on N-myc amplification within 48 hours, a regular protocol of treatment could be started without delay. We report that competitive PCR is a rapid and accurate method for the determination of the N-myc copy number, requiring only a small amount of material, and anticipate that competitive PCR will become the procedure of choice for the determination of N-myc copy number in neuroblastoma.
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