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  • Title: Expression of transforming growth factor (TGF)-beta1, TGF-beta2, and TGF-beta3 and of type I and II TGF-beta receptors during the development of the human fetal ovary.
    Author: Schilling B, Yeh J.
    Journal: Fertil Steril; 1999 Jul; 72(1):147-53. PubMed ID: 10428164.
    Abstract:
    OBJECTIVE: To investigate the role of transforming growth factor-beta (TGF-beta) in the regulation of human fetal ovarian development. DESIGN: Reverse transcription-polymerase chain reaction and comparative immunohistochemical analysis of the localization and staining intensity of TGF-beta1, TGF-beta2, and TGF-beta3, and of their receptors. SETTING: Academic research environment. PATIENT(S): Human fetal ovaries were obtained from terminated normal intact pregnancies at 11-24 weeks' gestation. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Messenger RNA analysis and protein expression of TGF-beta isoforms and their receptors in human fetal ovaries at 11-24 weeks of gestational age. RESULT(S): Messenger RNAs for the three TGF-beta isoforms and the two TGF-beta receptors were demonstrated in all the developmental ages studied: 11, 14, 18, 20, and 22 weeks of gestation. During the first trimester, immunohistochemical analysis for TGF-beta1, TGF-beta2, and TGF-beta receptor type I revealed homogeneous light staining of the ovary. Staining for TGF-beta3 and TGF-beta receptor type II was predominantly in the oocytes. During the second trimester, staining for all three TGF-beta isoforms and both receptors was predominantly in the oocytes. In addition, for receptor types I and II, staining was observed in the pregranulosa cells. CONCLUSION(S): Our findings support the hypothesis that expression of the TGF-beta system changes from the first to the second trimester of fetal development and may have an autocrine and/or paracrine regulatory role during ovarian development.
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