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  • Title: Chloramphenicol-sensitive mitochondrial translation in Trypanosoma brucei.
    Author: Nabholz CE, Speijer D, Schneider A.
    Journal: Parasitol Res; 1999 Aug; 85(8-9):779-82. PubMed ID: 10431749.
    Abstract:
    We developed an in organello system to label newly synthesized mitochondrially encoded proteins of Trypanosoma brucei. Highly purified mitochondria, prepared under isotonic conditions, were incubated with radioactive methionine and cysteine in a suitable translation buffer. Analysis of mitochondrial extracts on TRIS-Tricine gels revealed a subset of labeled, NP-40-insoluble proteins. The labeling of these proteins was resistant to the cytosol-specific translation inhibitor cycloheximide. The proteins, however, were not labeled in the presence of chloramphenicol or erythromycin, inhibitors of prokaryotic type translation, or puromycin, a general translation inhibitor. These results indicate that isotonically isolated mitochondria of T. brucei are capable of protein synthesis.
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