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  • Title: Influence of disulfide bonds on the induction of helical conformation in proteins.
    Author: Sivaraman T, Kumar TK, Hung KW, Yu C.
    Journal: J Protein Chem; 1999 May; 18(4):481-8. PubMed ID: 10449045.
    Abstract:
    The effect(s) of TFE (2,2,2-trifluoroethanol) on three different conformational states (native, denatured, and carboxymethylated) of CTX III and RNase A has been examined. Contrary to the general belief, the results of the present study reveal that TFE can induce helical conformation in a protein which has no sequence propensity to form a helix. It is found that the helix induction in TFE is intricately related to the destabilization of the tertiary structural conformation in proteins. More importantly, the disulfide bonds in proteins are found to have significant influence on the TFE-mediated helix induction. The results obtained in this study strongly suggest that information pertaining to the influence of disulfide bonds on helix induction need to be considered to improve the accuracy of secondary structure prediction algorithms.
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