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  • Title: [Efficient expression of human C1-inhibitor in CHO cells by using a dicistronic expression vector].
    Author: Yang H, Zheng S.
    Journal: Zhongguo Yi Xue Ke Xue Yuan Xue Bao; 1997 Feb; 19(1):41-7. PubMed ID: 10453551.
    Abstract:
    The full-length cDNA fragment encoding human C1-INH was obtained by gene recombination techniques and a stable expression plasmid was constructed by inserting the human C1-INH cDNA into an efficient dicistronic expression vector pED. This plasmid was transfected into DHFR-deficient Chinese Hamster Ovary cells (CHO-dhfr-) by Lipofectin method, and the positive CHO-dhfr+ cell clones which stably express C1-INH was generated. Expression of C1-INH mRNA was detected by Northern blot. Expression of C1-INH protein was confirmed by immunoprecipitation and Western blot analysis. The level of expressed C1-INH was estimated at 0.4 microgram/ml by specific ELISA techniques. The biological activity of recombinant C1-INH was assayed by using the inhibition of C1 estrolytic activity.
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