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  • Title: LIAISON hCG--an automated chemiluminescent immunoassay for the determination of human chorionic gonadotropin (hCG).
    Author: Oed M, Amtmann R, Löwer Y, Schlett R, Mack M.
    Journal: Anticancer Res; 1999; 19(4A):2735-7. PubMed ID: 10470231.
    Abstract:
    An immunoassay utilizing chemiluminescence and paramagnetic particles has been developed for the new fully automated, random access LIAISON immunoanalyzer. The LIAISON hCG assay is a two-site immunolouminometric one-step assay using two highly specific monoclonal antibodies. Total incubation time is only 10 min. A specially designed unique Reagent Integral contains all specific reagents; the on-board stability of these reagents is given over a very long period (> 4 weeks). The assay works with a 2-point calibrated master curve. 30 microliters sample is added to 200 microliters tracer and 20 microliters antibody-coated magnetic particles. After 10 min incubation the particles are separated, washed and the chemiluminescent signal is generated. The time to first result is only 15 min. The assay with a unique extended standard range up to 5,000 ng/ml shows no high dose hook effect up to 500,000 mlU/ml (spiked sera). The assay detects both the intact molecule and the free beta subunit. The cross-reactivity to FSH, LH and TSH is less than 0.1%. Precision (within-run < 3%; between-run < 5%), linearity, recovery and sensitivity (< 0.5 mlU/ml) are excellent. The assay shows a very good correlation to the LIA-mat hCG (r = 0.996). In summary the LIAISON hCG assay together with the new LIAISON immunoanalyzer is a very rapid and accurate method for the quantitative determination of hCG/beta-hCG in serum.
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