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Title: Cloning and sequencing of the bottle-nosed dolphin (Tursiops truncatus) interferon-gamma gene. Author: Inoue Y, Itou T, Oike T, Sakai T. Journal: J Vet Med Sci; 1999 Aug; 61(8):939-42. PubMed ID: 10487235. Abstract: The bottle-nosed dolphin (Tursiops truncatus) interferon-gamma(IFN-gamma) cDNA was cloned from mitogen stimulated peripheral blood mononuclear cells (PBMC) RNA utilizing the reverse transcription-polymerase chain reaction (RT-PCR). The dolphin IFN-gammacDNA was 585 base pairs in length and contained an open reading frame of 498 nucleotides encoding an IFN-gammaprecursor of 166 amino acids, with cleavage of the putative signal peptide of 19 amino acids. Analysis of the mature amino acid sequences revealed two potential N-linked glycosylation sites. Comparison of the predicted amino acid sequence showed that dolphin IFN-(gamma)shares 86, 85, 63 and 42% similarity with the bovine, ovine, human and mouse IFN-gamma, respectively.[Abstract] [Full Text] [Related] [New Search]