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  • Title: Comparison of preservation mixtures for Helicobacter pylori.
    Author: Liaw SJ, Teng LJ, Ho SW, Luh KT.
    Journal: J Microbiol Immunol Infect; 1998 Dec; 31(4):261-3. PubMed ID: 10496170.
    Abstract:
    Long-term preservation of 40 isolates of Helicobacter pylori was investigated with five storage media (brucella broth with 17% glycerol, brucella broth with 17% glycerol and 2% fetal calf serum [FCS], brucella broth with 17% glycerol and 10% FCS, brucella broth with 17% glycerol and 2% horse blood, and 10% mucin) at -70 degrees C for 4, 6 and 9 months. In addition to glycerol, FCS or horse blood in the storage media is necessary for survival of H. pylori. Storage of H. pylori isolates at -70 degrees C in 10% mucin is a simple and effective preservation procedure.
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