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  • Title: Multiple calcium channels regulate neurotransmitter release from vagus nerve terminals in the cat bronchiole.
    Author: Fujisawa K, Onoue H, Abe K, Ito Y.
    Journal: Br J Pharmacol; 1999 Sep; 128(1):262-8. PubMed ID: 10498861.
    Abstract:
    1. Twitch-like contractions and non-adrenergic non-cholinergic (NANC) relaxations evoked by electrical field stimulation (EFS) of the cat bronchiole were used to examine the voltage-activated calcium channels involved in excitatory and inhibitory neurotransmission in the cat bronchiole. 2. Nifedipine (50 microM), the L-type calcium channel antagonist, did not affect the twitch-like contraction and NANC relaxations. However, low concentrations of the N-type calcium channel blocker omega-conotoxin GVIA (omega-CgTX GVIA) (0.1 microM) irreversibly abolished twitch-like contractions evoked by trains of EFS </=10 stimuli at 20 Hz. 3. After the prolonged treatment with 0.1 microM omega-CgTX GVIA, EFS evoked initial fast and later slow NANC relaxations in the presence of 5-HT (10 microM), atropine and guanethidine (1 microM each). However increased concentration of omega-CgTX GVIA (1 microM) completely suppressed the slow NANC relaxation without affecting the initial fast component. 4. omega-agatoxin IVA (100 nM), the P- and Q-type calcium channel inhibitor, and nimodipine (10 microM), the L- and T-type calcium channel blocker, did not affect the amplitude of the initial fast NANC relaxation in the absence or presence of omega-CgTX GVIA (1 microM). 5. The contraction or relaxation induced by exogenous acetylcholine (ACh) (0.5 microM) or the nitric oxide donor, s-nitroso-N-acetyl penicillamine (SNAP) (1 microM) were not affected by omega-CgTX GVIA (1 microM). 6. Taken together, these results suggest that generation of twitch-like contraction and later slow NANC relaxation are regulated by N-type calcium channels, whereas generation of the initial fast NANC relaxation possibly involves R-type calcium channel.
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