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  • Title: Aptamer affinity chromatography: combinatorial chemistry applied to protein purification.
    Author: Romig TS, Bell C, Drolet DW.
    Journal: J Chromatogr B Biomed Sci Appl; 1999 Aug 20; 731(2):275-84. PubMed ID: 10510781.
    Abstract:
    The systematic evolution of ligands by exponential enrichment process is a combinatorial chemistry method that allows the identification of specific oligonucleotide sequences, known as aptamers, that bind to a desired target molecule with high affinity and specificity. Here, a DNA-aptamer specific for human L-selectin was immobilized to a chromatography support to create an affinity column. This column was effectively applied as either the first or second step in the purification of a recombinant human L-selectin-Ig fusion protein from Chinese hamster ovary cell-conditioned medium. The fusion protein was efficiently bound to the column and efficiently eluted by gentle elution schemes. Application of the aptamer column as the initial purification step resulted in a 1500-fold purification with an 83% single step recovery. These results demonstrate that oligonucleotide aptamers can be effective affinity purification reagents.
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