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  • Title: Antioxidant supplementation in vitro does not improve human sperm motility.
    Author: Donnelly ET, McClure N, Lewis SE.
    Journal: Fertil Steril; 1999 Sep; 72(3):484-95. PubMed ID: 10519621.
    Abstract:
    OBJECTIVE: To determine the effects of supplementation of preparation media with ascorbate and alpha-tocopherol on subsequent sperm motility and reactive oxygen species production. DESIGN: Prospective study to analyze postpreparation human sperm motility parameters and reactive oxygen species production following antioxidant supplementation. SETTING: Andrology Laboratory, Royal Maternity Hospital, Belfast, Northern Ireland. PATIENT(S): Sixty patients attending the Andrology Laboratory for semen analysis. INTERVENTION(S): Normozoospermic and asthenozoospermic semen samples (n = 10 for each control and antioxidant group) were prepared by Percoll density centrifugation (95%-47.5%) in media supplemented with ascorbate or alpha-tocopherol to different concentrations within physiologic levels. Controls were included that were not exposed to antioxidant. MAIN OUTCOME MEASURE(S): Sperm motility parameters were assessed using computer-assisted semen analysis. The generation of reactive oxygen species was determined using luminol-dependent chemiluminescence. RESULT(S): The production of reactive oxygen species by sperm was reduced by supplementation in vitro with ascorbate and alpha-tocopherol. However, progressive motility, average path velocity, curvilinear velocity, straight-line velocity, and linearity were decreased significantly, with the greatest inhibition observed with the highest concentrations of antioxidants. CONCLUSION(S): Supplementation of preparation media with ascorbate and alpha-tocopherol, either singly or in combination, is not beneficial to sperm motility.
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