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  • Title: Assembly of chick brain tubulin onto flagellar microtubules from Chlamydomonas and sea urchin sperm.
    Author: Binder LI, Dentler WL, Rosenbaum JL.
    Journal: Proc Natl Acad Sci U S A; 1975 Mar; 72(3):1122-6. PubMed ID: 1055370.
    Abstract:
    Flagellar microtubules from Chlamydomonas and sea urchin sperm were used as in vitro assembly sites for chick brain tubulin. Brain microtubules assembled onto the A-tubules, central tubules, and, to a limited extent, onto the distal ends of the axonemes at low tubulin concentrations and onto distal and proximal ends at high tubulin concentrations; however, the rate of assembly onto the distal end was always greater. The rate of neurotubule assembly onto axonemes was shown to be dependent upon tubulin concentration and a forward rate constant for assembly was determined.
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