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  • Title: Differentiation-induced insulin secretion from nonendocrine cells with engineered human proinsulin cDNA.
    Author: Yamasaki K, Sasaki T, Nemoto M, Eto Y, Tajima N.
    Journal: Biochem Biophys Res Commun; 1999 Nov 19; 265(2):361-5. PubMed ID: 10558872.
    Abstract:
    To investigate the effects of differentiation on insulin secretion from engineered nonendocrine cells, modified human proinsulin cDNA (INS/fur) was transfected to THP-1 monocyte and C2C12 myoblast cell lines. When THP-1 was differentiated into macrophages with phorbol ester, the insulin secretion rate was increased by 3.1-fold. This increase in insulin secretion is accompanied by a 17.6-fold increase in the processing efficiency of the modified human proinsulin and by a 3.5-fold increase in the abundance of furin mRNA. In addition, differentiation of C2C12 into myotubes, which can be induced by changing the serum, showed a 9.9-fold increase in insulin secretion and was accompanied by a 1.6-fold increase in the abundance of furin mRNA. The involvement of posttranslational processing and the exocytotic process in differentiation-induced insulin secretion could lead to the possibility of regulation of insulin secretion from genetically engineered cells.
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