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  • Title: Adenosine inhibits a non-inactivating K+ current in bovine adrenal cortical cells by activation of multiple P1 receptors.
    Author: Xu L, Enyeart JJ.
    Journal: J Physiol; 1999 Nov 15; 521 Pt 1(Pt 1):81-97. PubMed ID: 10562336.
    Abstract:
    1. Bovine adrenal zona fasciculata (AZF) cells express a non-inactivating K+ current (IAC) that sets the resting potential while it is activated by intracellular ATP. In whole-cell patch clamp recordings from bovine AZF cells, we found that adenosine selectively inhibited IAC by a maximum of 78.4 +/- 4.6 % (n = 8) with an IC50 of 71 nM. The non-selective adenosine receptor agonist NECA effectively inhibited IAC by 79.3 +/- 2.9 % (n = 24) at a concentration of 100 nM. 2. Inhibition of IAC was mediated through multiple P1 adenosine receptor subtypes. The A1-selective agonist CCPA (10 nM), the A2A-selective agonist CGS 21680 (100 nM) and the A3-selective agonist IB-MECA (10 nM) inhibited IAC by 64.8 +/- 8.4, 78.4 +/- 4.6 and 69.3 +/- 6.9 %, respectively. 3. Specific adenosine receptor subtype antagonists including DPCPX (A1), ZM 241385 (A2A) and MRS 1191 (A3) effectively blocked inhibition of IAC by adenosine receptor-selective agonists. 4. A mixture of the three adenosine receptor antagonists completely suppressed inhibition of IAC by adenosine, but failed to alter inhibition by external ATP which acts through a separate P2 nucleotide receptor. 5. Inhibition of IAC by adenosine or NECA was eliminated by substituting GDP-beta-S for GTP in the pipette, or by replacing ATP with AMP-PNP or UTP. 6. In addition to inhibiting IAC, adenosine (10 microM) depolarized AZF cells by 46.2 +/- 5.8 mV (n = 6). 7. These results show that bovine AZF cells express at least three adenosine receptor subtypes (A1, A2A, A3), each of which is coupled to the inhibition of IAC K+ channels through a G-protein-dependent mechanism requiring ATP hydrolysis. Adenosine-mediated inhibition of IAC is associated with membrane depolarization. Adenosine and other purines may co-ordinate the stress-induced secretion of corticosteroids and catecholamines from the adrenal gland.
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