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  • Title: Visualization of actin fibers associated with the cell membrane in amoebae of Dictyostelium discoideum.
    Author: Clarke M, Schatten G, Mazia D, Spudich JA.
    Journal: Proc Natl Acad Sci U S A; 1975 May; 72(5):1758-62. PubMed ID: 1057168.
    Abstract:
    Amoebae of Dictyostelium discoideum were attached to a surface coated with polylysine, and the upper portion of the cells was sheared off with a stream of buffer. Scanning and transmission electron microscopy showed that the cytoplasmic surface of the exposed membrane was covered with fibers consisting of actin-containing filaments. The actin was identified by its solubility properties and its ability to interact with muscle myosin.
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