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  • Title: Carbohydrate release from picomole quantities of glycoprotein and characterisation of glycans by high-performance liquid chromatography and mass spectrometry.
    Author: Charlwood J, Birrell H, Camilleri P.
    Journal: J Chromatogr B Biomed Sci Appl; 1999 Oct 29; 734(1):169-74. PubMed ID: 10574203.
    Abstract:
    Samples of 5 to 20 microg of human IgG were subjected to dithiothreitol treatment to reduce disulphide bridges, followed by tryptic digestion. Glycans released from the tryptic peptide mixture by PNGase F digestion were then derivatised with 2-aminoacridone. Labelled oligosaccharides were separated by normal-phase high-performance liquid chromatography and individual components were collected for matrix-assisted laser desorption ionization time-of-flight and electrospray mass spectrometric analysis.
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