These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: elt-1, a gene encoding a Caenorhabditis elegans GATA transcription factor, is highly expressed in the germ lines with msp genes as the potential targets.
    Author: Shim YH.
    Journal: Mol Cells; 1999 Oct 31; 9(5):535-41. PubMed ID: 10597043.
    Abstract:
    The Caenorhabditis elegans ELT-1 protein, a homolog of the vertebrate GATA transcription factor family, is a transcription activator that can recognize the GATA motif. We previously showed that the elt-1 mRNA was primarily expressed in C. elegans embryos. To examine whether the elt-1 mRNA in embryos is maternal, paternal or zygotic, Northern blot analysis was performed with RNA isolated from the C. elegans germ-line mutant strains, fem-2 (b245)lf, fem-3 (q20)gf, him-8 (e1489), and glp-4 (bn2). This analysis revealed that the high level of elt-1 mRNA in the C. elegans embryos resulted from either the maternal or the paternal transcription, rather than from the zygotic expression. These results further demonstrated that elt-1 was highly expressed in the germ-line of both sexes. To investigate the possible target genes for the ELT-1 protein in the germ line, the ELT-1 protein was expressed and tested for its binding specificity to the GATA motif that is present in the promoter region of the C. elegans major sperm protein genes. It was found that two conserved cis-elements, AGATCT and AGATAA, in the proximal promoter region of the msp-113 gene provided the best recognition site for ELT-1. Mutational analysis showed that the GATC core sequence was necessary for strong transactivation of the reporter gene, and that the combination of GATC and GATA motif resulted in a stronger transactivation by ELT-1 than either the duplicated GATC or GATA motif. These results suggest that the potential target for the ELT-1 protein in the germ-line may be one of the major sperm protein gene family.
    [Abstract] [Full Text] [Related] [New Search]