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  • Title: Assignment of cytosine N3 resonances in nucleic acids via intrabase three-bond coupling to amino protons.
    Author: Rüdisser S, Pelton JG, Tinoco I.
    Journal: J Biomol NMR; 1999 Oct; 15(2):173-6. PubMed ID: 10605090.
    Abstract:
    Coherences were observed between 15N3 of cytosine and its trans amino proton (H42) using a modified gradient-based heteronuclear single quantum coherence (HSQC) pulse sequence optimized for three-bond proton-nitrogen couplings. The method is demonstrated with a 22-nucleotide RNA fragment of the P5abc region of a group I intron uniformly labeled with 15N. Use of intraresidue 15N3-amino proton couplings to assign cytosine 15N3 signals complements the recently proposed JNN HNN COSY [Dingley, A.J. and Grzesiek, S. (1998) J. Am. Chem. Soc., 120, 8293-8297] method of identifying hydrogen-bonded base pairs in RNA.
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