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  • Title: Serological and molecular diagnosis of bovine viral diarrhoea virus and evidence of other viral infections in dairy calves with respiratory disease in Venezuela.
    Author: Obando C, Baule C, Pedrique C, Veracierta C, Belák S, Merza M, Moreno-Lopez J.
    Journal: Acta Vet Scand; 1999; 40(3):253-62. PubMed ID: 10605142.
    Abstract:
    An investigation based on 2 studies was carried out to assess the involvement of bovine virus diarrhoea virus (BVDV), bovine herpesvirus type 1 (BHV-1), and bovine respiratory syncytial virus (BRSV) in calf respiratory disease in dairy farms in Venezuela. In the first study, 8 farms were selected and paired serum samples from 42 calves with respiratory disease were tested by ELISA for antibodies to the 3 viruses. Seroconversion to BVDV, BHV-1, and BRSV was found to 5, 2, and 6 farms out of the 8, respectively. The proportion of calves that showed seroconversion to BVDV, BHV-1, and BRSV were 19%, 14%, and 26%, respectively. In the second study, another farm having previous serological evidence of BVDV infection was selected. The decline of maternal antibodies against BVDV was monitored in 20 calves and the half-life of maternal antibodies was 34 +/- 12 days presumably indicating an early natural infection with BVDV. Furthermore, sera free of BVDV antibodies that were collected in studies 1 and 2 and were assayed for the presence of BVDV by nested RT-PCR. Two BVDV strains were detected and compared to those of ruminant and porcine pestiviruses. Both strains were assigned to subgroup Ib of type I BVDV. This investigation provides information on BVDV genotypes circulating in Venezuela and may contribute to the establishment of official control programmes against the viruses studied. Två studier genomfördes for att påvisa bovint virus diarré virus (BVDV), bovint herpesvirus typ 1 (BHV-1) och bovint respiratoriskt syncytial virus (BRSV) hos kalvar med luftvägsinfektioner. I den första studier, parserum från 42 kalvar i åtta besättningar testades med ELISA for antikroppar mot de 3 virus. Serokonverteringar for BVDV, BHV-1 och BRSV kunde påvisas i 5, 2 och 6 av respektive 8 besättningar. Procentuellt sätt kalvarna som serokonverterade vid BVDV, BHV-1 och BRSV var 19, 14 och 26 procent till respektiva virus. I den andra studier en ny besättningen med BVDV problem selekterades och halvering av maternala antikroppstiter mot BVDV undersöktes i 20 kalvar. Titrarna halverades efter 34 ± 12 dagar som indikerar en tidigt infektion med BVDV Dessutom BVDV fri sera från djur I studier 1–2 testades med RT-PCR för att påvisa närvaro av BVDV-RNA. Två BVDV stammar av typ I subgrup IB detekterades. Resultaterna indikerar att BVDV genotyper cirkulerar i Venezuela och resultaterna kommer att bidra till genomförande av officiell kontroll av de undersökta virus.
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