These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Polypeptide chain initiation in eukaryotes: initiation factor requirements for translation of natural messengers.
    Author: Filipowicz W, Sierra JM, Nombela C, Ochoa S, Merrick WC, Anderson WF.
    Journal: Proc Natl Acad Sci U S A; 1976 Jan; 73(1):44-8. PubMed ID: 1061126.
    Abstract:
    A protein-synthesizing system consisting of ribosomes and supernatant of undeveloped Artemia saline embryos is used for assay of mRNA translation and initiation factors. This system contains the components needed for chain elongation but has low levels of mRNA and initiation factors. Exogenous mRNA is readily translated upon addition of high-speed supernatant or ribosomal salt wash of developing embryos as a source of initiation factors (IF). This requirement can be largely satisfied by a mixture of the reticulocyte factors IF-MP, IF-M3, IF-M2A, and IF-M2B. A. salina IF-M1, which is present in undeveloped embryo supernatant, can be inactivated by A. salina IF-M1 antibody without affecting translation.
    [Abstract] [Full Text] [Related] [New Search]