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  • Title: Functional expression of His-tagged sensory rhodopsin I in Escherichia coli.
    Author: Schmies G, Chizhov I, Engelhard M.
    Journal: FEBS Lett; 2000 Jan 21; 466(1):67-9. PubMed ID: 10648814.
    Abstract:
    Sensory rhodopsin I (SRI) from Halobacterium salinarum was functionally expressed in Escherichia coli and subsequently purified to homogeneity using a C-terminal His-tag anchor. Yields of 3-4 mg SRI/l cell culture can be obtained. The absorption and photocycle properties of SRI were similar if not indistinguishable from those of the homologously expressed SRI. A global fit analysis of the photocycle data and the calculation of the spectra of states provided strong evidence for the existence of an N-like intermediate.
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